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KMID : 1023620080320040245
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Reproductive and Developmental Biology
2008 Volume.32 No. 4 p.245 ~ p.248
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Effects of Viability of Canine Oocytes Vitrified-Warmed by the EDS and EDT Methods
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Park Ji-Hoon
Yasuyuki Abe
Kim Sang-Keun
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Abstract
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In the present studies, we have intended to compare the EDS (20% EG + 20% DMSO + 0.4 M sucrose + 10% FCS) and EDT (20% EG + 20% DMSO + 0.3 M trehalose 10% FCS) methods for vitrification of canine oocytes, in order to improve the vitrification methods. The survival rate of vitrified-thawed oocytes using the EDS method was (p<0.05), which was lower than that of the control group . About of the vitrified-warmed oocytes showed normal morphology, as assessed by PI staining. However, the ratio of survival rate of oocytes showed lower than that of normal morphology in comparison between EDS method and control group. The survival and developmental rates of vitrified-warmed oocytes by the EDS and EDT methods were and , respectively (p<0.05). The results were significantly lower than the control group . However, the survival rate of vitrified-warmed oocytes using EDS method showed higher than that in the ETS group.
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KEYWORD
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Canine oocytes, Vitrification, Survival rate, EDS, EDT methods
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