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KMID : 1023620100340030241
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Reproductive and Developmental Biology
2010 Volume.34 No. 3 p.241 ~ p.246
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Effect of BTS and Androhep during Storage Times on the Kinematics and Capacitation Status in Liquid Boar Semen
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Kim Yeon-Hee
Park Yoo-Jin
Yoon Sung-Jae
Kwon Woo-Sun
Kim Sang-Hyun
Park Myung-Geol
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Abstract
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The objective of this study was to investigate the effect of storage time on fresh boar semen in Androhep and Beltsville Thawing Solution (BTS). Boar semen samples extended in each extender were stored at 17℃ up to 4 days. Sperm motility kinematics was evaluated by computer assisted sperm analyzer (CASA) and capacitation status by chlortetracycline (CTC)/Hoechst 33258 staining. Sperm motility (%) was not decreased during storage in BTS and Androhep. No significant difference between extenders was observed. Only significant differences in kinematic parameters on linearity during storage were found. The percentage of dead sperm significantly decreased during storage (p<0.05). Also the percentage of noncapacitated, capacitated, and acrosome-reacted sperm significantly modified during storage (p<0.05). However, there was no significant difference between extenders except proportion of capacitated sperm. This finding supported that modification in these parameters was not significantly different between extenders during this short-term storage. Our finding strongly indicated that both Androhep and BTS maintained favorable conditions for motility, motility kinematics, and capacitation status during short-term storage. Despite modifications in some parameters were apparent during sperm storage in extenders, these may not affect the fertilizing capacity of boar semen.
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KEYWORD
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Boar, Semen storage, BTS, Androhep, Motility kinematics, Capacitation
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