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KMID : 1023620120360010001
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Reproductive and Developmental Biology
2012 Volume.36 No. 1 p.1 ~ p.6
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Analysis of Sexed Sperm by Flow Cytometry in Hanwoo (Korean Native Cattle)
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Yoo Han-Jun
Lee Kyung-Jin
Lee Yong-Seung
Yoon Pil-Sang
Park Joung-Jun
Kim Hyeong-Cheol
Park Choon-Keun
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Abstract
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This study evaluated a sexed sperm ability to produce embryos by flow cytometer. Hanwoo bulls sperm were
separated to X and Y sperm via Hoechst 33342 stained with near UV laser or performed the pre-sorted without near
UV laser beam in flow cytometry. Pre-sorted sperm had significantly higher viability (84±1.15 %, p<0.05) compared
to other sorted groups in frozen-thawed semen. For fresh semen, pre-sorted sperm had the higher viability (79±3 %,
p<0.05) than those of the X and Y sperm (44.7±1.67 and 41.7±1.2 %) separated by differences of DNA content. On the
other hand, pre-sorted and X sperm sorted according to differences in DNA content had significantly higher viabilities
(24.3±1.2 and 25.7±0.9 %, p<0.05) compared to that of the sorted Y sperm (13.7±1.2 %) in the hypoosmotic swelling
test. The proportion acrosome reaction in the sorted X sperm was higher (55.0±1.7 and 45.0±1.5 %) than those of the
sorted Y-sperm (32.3±0.9 %, p<0.05). However, the sperm morphologies of the sorted groups were not significantly
differences. In conclusion, the sex-sorting procedure by flow cytometry affected some characteristics of Hanwoo sperm.
Further study is needed to determine the optimal procedures to enhance male and female embryos and sorting
accuracy.
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KEYWORD
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Flow cytometry, Sperm sorting, Sperm analysis, Hanwoo
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