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KMID : 1023620120360010027
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Reproductive and Developmental Biology
2012 Volume.36 No. 1 p.27 ~ p.32
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Analysis on Artificial Insemination Failure and Characteristics of Frozen Semen Used for Reproduction of Hanwoo Cow in Gangwon East Area
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Park Sai-Rom
Hong Min-Wook
Kim Hun
Lee Seung-Kyu
Lee Yeung-Sub
Kim J. W.
Lee Hak-Kyo
Jeong Dong-Kee
Kim Jong-Bok
Song Young-Han
Lee Sung-Jin
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Abstract
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This study was carried out to investigate artificial insemination (AI) failure status and frozen semen characteristics in Korean proven bulls‘ number (KPN) semen used for AI of Hanwoo cows in Gangwon East region (Gangneung, Donghae, Taebaek, Samcheok, Sokcho, Yangyang, Goseong). Among semen used for AI, AI failure rate showed lowest at KPN506 (27.6%), whereas highest at KPN593 (77.2%). Correlations of AI failure in between Korean proven bulls semen and cows was 0.2941, which means that AI failure rate of Korean proven bulls semen may have respectableeffect on reproduction of Hanwoo cow. In addition, present study was conducted to investigate spermatozoal viability rate, ruptured acrosome rate and active mitochondria in frozen Korean proven bulls semen with flow cytometry. The semen of KPN593 showed significantly (p<0.05) higher viability rate in KPN593 (30.49%) than that in KPN637 (37.34%). Furthermore, percentage of ruptured acrosome was lower in KPN637 as 21.37% than in KPN637 (21.37%), but it was not statistically significant. In conclusion, these results indicate that choice of Korean proven bulls semen may correlate positively with conception rate in Hanwoo cow. Therefore, KPN with high AI failure rate might be avoid to increase conception rate and characteristics of frozen semen might be evaluated before its use for AI.
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KEYWORD
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Artificial insemination, KPN semen, Hanwoo cows, Flow-cytometry
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