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KMID : 1023620120360030219
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Reproductive and Developmental Biology
2012 Volume.36 No. 3 p.219 ~ p.223
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Relationship between Plasminogen Activator (PA) and HSP-90 in Uterus Tissue during the Porcine Estrous Cycle
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Gu Ha-Na
Cheong Hee-Tae
Yang Boo-Keun
Park Choon-Keun
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Abstract
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The present study was performed to identify the relationship between plasminogen activator (PA) and Heat Shock Protein-90 (HSP-90) in porcine uterus tissues during the estrous cycle. Porcine uterus tissues were obtained from preovulatory (Pre-Ov), post-ovulatory (Post-Ov) and early to mid-luteal (Early-mid L) stages. The protein was extracted from uterus tissue by using M-PER Mammalian Protein Extraction Reagent. Proteins were refined by RIPA Buffer and quantified by BCA methods. As results, t-PA expression was significantly (p<0.05) higher from pre-ovulatory(Epithelium tissue: 29,067㎛/μl, Myometrium tissue: 30,797㎛/μl) compared to the post-ovulatory stage(Epithelium tissue: 54,357㎛/μl, Myometrium tissue: 53,270㎛/μl) and early to mid-luteal stage(Epithelium tissue: 42,380㎛/μl, Myometrium tissue: 43,139㎛/μl). On the other hand, the uPA expression indicated higher from early to mid-luteal stage (Epithelium tissue: 0.02198㎛/μl, Myometrium tissue: 0.02412㎛/μl) than pre-ovulatory stage (Epithelium tissue: 0.01577㎛/μl, Myometrium tissue: 0.01531㎛/μl) and post-ovulatory stage(Epithelium tissue: 0.01414㎛/μl, Myometrium tissue: 0.01429㎛/μl). However, expression of u-PA did not differ from each estrous cycle in the epithelium tissue and myometrium tissue(p<0.05). Expression of HSP-90 was differ t-PA and u-PA from pre-ovulatory in Epithelium tissue(25,423㎛/μl) and early to mid-luteal stage in epithelium tissue(177,922㎛/μl) and myometrium tissue(26,664㎛/μl). These results suggest that HSP-90 and u-PA were related with change of uterus cycle according to the reformation of the tissues in porcine uterus.
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KEYWORD
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Porcine estrous cycle, Heat shock protein-90, Tissue-plsminogen activator (t-PA), Urokinase-plsminogen activator (u-PA), Uterus tissue
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