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KMID : 1023620130370040219
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Reproductive and Developmental Biology
2013 Volume.37 No. 4 p.219 ~ p.224
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Comparison of Gene Expression Levels of Porcine Satellite Cells from Postnatal Muscle Tissue during Differentiation
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Jeong Jin-Young
Kim Jang-Mi
Rajesh Ramanna Valmiki
Suresh Sekar
Jang Gul-Won
Lee Kyung-Tai
Kim Tae-Hun
Park Mi-Na
Cho Yong-Min
Lee Hyun-Jeong
Jeong Hak-Jae
Kim Kyung-Woon
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Abstract
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Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson’s, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma (PPARr) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.
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KEYWORD
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Porcine satellite cell, Adipogenesis, Myogenesis, Osteoblastogenesis, Differentiation
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