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KMID : 1023620130370040255
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Reproductive and Developmental Biology
2013 Volume.37 No. 4 p.255 ~ p.261
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A Proteome Reference Map for Porcine Plasma Proteins
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Jeong Jin-Young
Nam Jin-Sun
Park Mi-Rim
Kim Jang-Mi
Jeong Hak-Jae
Kim Kyung-Woon
Lee Hyun-Jeong
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Abstract
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To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.
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KEYWORD
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2-DE, Proteome, Porcine, MALDI-TOF-TOF, LC-MS-MS
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