KMID : 1023820110030030152
|
|
The Journal of Advanced Prosthodontics 2011 Volume.3 No. 3 p.152 ~ p.160
|
|
Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation
|
|
Huh Jung-Bo
Lee Jeong-Yol Lee Kyung-Lae Kim Sung-Eun Yun Mi-Jung Shim June-Sung Shin Sang-Wan
|
|
Abstract
|
|
|
PURPOSE : This study was to investigate the effects of recombinant human platelet-derived growth factor (rhPDGF-BB) and heparin to titanium surfaces for enhancement of osteoblastic functions and inhibition of inflammation activity.
MATERIALS AND METHODS : The anodized titanium discs, not coated with any material, were used as a control group. In heparinized- Ti group, dopamine was anchored to the surface of Ti substrates, and coated with heparin. In PDGF-Ti group, rhPDGF-BB was immobilized onto heparinized Ti surface. The surface morphologies were investigated by the scanning electron microscope in each group. The release kinetics of rhPDGF-BB were analyzed, and cytotoxicity tests for each group were conducted. The biocompatibilities were characterized by measuring cell proliferation, alkaline phosphatase activity, and calcium deposition using MG-63 cells. Statistical comparisons were carried out by one-way ANOVA tests. Differences were considered statistically significant at *P<.05 and **P<.001.
RESULTS : The combination of rhPDGF-BB and heparin stimulated alkaline phosphatase activity and OCN mRNA expression in osteoblastic cells (*P<.05 and **P<.001). MG-63 cells grown on PDGF-Ti had significantly higher amounts of calcium deposition than those grown on anodized Ti (**P<.001). Heparinized Ti was more anti-inflammatory compared to anodized Ti, when exposed to lipopolysaccharide using the transcript levels of TNF-¥á and IL-6 of proinflammatory cytokine (*P<.05 and **P<.001).
CONCLUSION : The result of this study demonstrated that the incorporation of rhPDGF-BB and heparin onto Ti surface enhanced osteoblastic functions and inhibited inflammation.
|
|
KEYWORD
|
|
Titanium, Heparin, rhPDGF-BB, Anti-inflammation, Osteoblastic function
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|