KMID : 1024220140290010018
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Environmental Health and Toxicology : EHT 2014 Volume.29 No. 1 p.18 ~ p.18
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The separation of arsenic metabolites in urine by high performance liquid chromatographyinductively coupled plasma-mass spectrometry
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:Chung Jin-Yong
:Lim Hyoun-Ju/:Kim Young-Jin/:Song Ki-Hoon/:Kim Byoung-Gwon/:Hong Young-Seoub
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Abstract
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Objectives: The purpose of this study was to determine a separation method for each arsenic metabolite in urine by using a high performance liquid chromatography (HPLC)- inductively coupled plasma-mass spectrometer (ICP-MS).
Methods: Separation of the arsenic metabolites was conducted in urine by using a polymeric anion-exchange (Hamilton PRP X-100, 4.6 mm¡¿150 mm, 5 ¥ìm) column on Agilent Technologies 1260 Infinity LC system coupled to Agilent Technologies 7700 series ICP/MS equipment using argon as the plasma gas.
Results: All five important arsenic metabolites in urine were separated within 16 minutes in the order of arsenobetaine, arsenite, dimethylarsinate, monomethylarsonate and arsenate with detection limits ranging from 0.15 to 0.27 ¥ìg/L (40 ¥ìL injection). We used GEQUAS No. 52, the German external quality assessment scheme and standard reference material 2669, National Institute of Standard and Technology, to validate our analyses.
Conclusions: The method for separation of arsenic metabolites in urine was established by using HPLC-ICP-MS. This method contributes to the evaluation of arsenic exposure, health effect assessment and other bio-monitoring studies for arsenic exposure in South Korea.
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KEYWORD
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Arsenic, Arsenic metabolites, HPLC-ICP-MS, Urine
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