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KMID : 1024520020110010033
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Journal of the Environmental Sciences
2002 Volume.11 No. 1 p.33 ~ p.40
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Production and Characteristics of Environment-Friendly Antimicrobial Substance by Pseudomonas aeruginosa EL-KM
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Lee Kyung-Min
Lee O-Mi
Cha Mi-Sun
Park Eun-Hee
Park Geun-Tae
Son Hong-Joo
Lee Sang-Joon
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Abstract
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An antimicrobial substance-producing microorganism was isolated from soil samples. Based of the taxonomic characteristics of its morphological, cultural, physiological properties and 16s rRNA sequence alignment, this microorganism was identified as Pseudomonas aeruginosa, and we named Pseudomonas aeruginosa EL-KM. The optimal culture condition for production of antimicrobial substance was 1% mannitol, 0.4% yeast extract, 0.5% Nacl, 0.2% , 100M ., 10M ., 1M ., 1M ., initial pH 7 and 200 rpm at 3. The purification of the antimicrbial substance was performed by silica gel column chromatographys, and fraction with TLC 0.77 value represented good antimicrobial activity. The crude antimicrbial substance was stable within a pH range of 3-10 and temperature range of 4-121 autoclaving. This crude antibacterial substance acted as bacteriolytic agent against Vibrio cholerae non-Ol ATCC 25872, and also exhibited excellent properties, when the substance was demonstrated against many other gram-positive, gram-negative bacteria, yeast and fungi.
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KEYWORD
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Antimicrobial substance, Pseudomonas aeruginosa EL-KM, Vibrio
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