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KMID : 1024619980180020185
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Food Science of Animal Resources
1998 Volume.18 No. 2 p.185 ~ p.191
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Enzymatic Activity and Distribution of Marker Enzymes between Human Milk and Bovine Milk with Their Separated Milk Fractions
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Cho Jin-Kuk
Takeda Y.
Kim Cheon-Jei
Kim Chang-Han
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Abstract
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Human milk and bovine milk in normal stage were fractionated four parts : whey, skimmilk membrane, and casein pellet. The specific activity (nmole / mim / mg protein) and distribution ratio(%) of suborganella marker enzymes in each separated milk fraction were determined. Especially, neutral -ATPase, acid -ATPase, NADH-cytochrome C reductase, and acid phosphatase were higher in human milk. However, both -ATPases were not detected in all fractions of bovine milk. On the other hand, 5’-nucleotidase, phosphodiesterase I, alkaline phosphatase, and -glutamyl transpeptidase activities in bovine milk were higher than in human milk. Most of the marker enzymes were highly distributed in cream fraction of either human milk or bovine milk, and their specific activities were high to 24 fold from 3 fold when compared with that of whole milk. These results suggest that marker enzymes in mammary epitherial cell are transfered into cream fraction by the membrane rearrangement, and different biochemical reaction between human and bovine exists for milk secretion in mammary gland.
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KEYWORD
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marker enzymes, human milk, bovine milk, MFGM
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