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KMID : 1024620000200020114
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Food Science of Animal Resources
2000 Volume.20 No. 2 p.114 ~ p.124
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Evaluation of Allergenicity for Fish and Method for Reduction of Allergenicity by Food Technological Treatment
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Lee Bou-Oung
Chang O. K.
Oh D. K.
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Abstract
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In this research the results showed that Evans blue stain causes vascular permeation at antibody injection site by the passive cutaneous anaphylaxis(PCA) screening of octpus minor so we concluded. Octopus minor causes allergy. Psedosciaena Polyactis, Raja Kenojei, Metapenaeus joyneri also showed allergenicity. Microwave and autoclaving appeared to reduced allergenicity(up to 99%) during the technological treatment processing. On the other hand, UV light didn’t seem to change the protein structure of allergens affect the allergenicity. Therefore, the technological treatment processing of fish such as canning and microwave would possibly reduce the allergenicity. Among the ultrafiltration fraction of Octopus minor, Pseudosciaena Polyactis, Raja Kenojei and Metapenaeus joyneri, those fraction over 100,000 contained allergen and those under 100,000 and when screening allergenic fish went through 10,000~100,000 ultrafiltration, only the fraction of over 100,000 showed the anaphylactis activity for PCA. However whether screening fish would cause anaphylaxis in human or not is questionable. The future clinical experiment will verify this result with clinical experiment patients.
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KEYWORD
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anaphylaxis, allergen, fish, molecular weights, PCA, reduction of allergy, treatment
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