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KMID : 1024620000200020125
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Food Science of Animal Resources
2000 Volume.20 No. 2 p.125 ~ p.132
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Isolation and Purification of Lactoferrin from Korean Native Cow’s Colostrum
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Yang H. J.
Ha W. K.
Yang D.-H.
Park K. M.
Lee Soo-Won
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Abstract
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Lactoferrin was isolated from the colostrum of Korean native cow by using several purification steps such as batch extraction, ion exchange chromatography, gel filtration chromatography and affinity chromatography. Other whey protein components that having similar molecular weight and affinity to lactoferrin were gradually removed from crude Korean native cow’s lactoferrin during the purification steps. The amount of lactoferrin collected from a liter of Korean native cow’s colostrum was 65mg and the recovery rate was 29.4%. The molecular weight of the purified Korean native cow’s lactoferrin was estimated approximately 81,000dalton.
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KEYWORD
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lactoferrin, Korean native cow’s colostrum, isolation and purification
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