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KMID : 1024620030230020155
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Food Science of Animal Resources
2003 Volume.23 No. 2 p.155 ~ p.160
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Studies of Purification and Characterization of Epidermal Growth Factor from Human Colostrum
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Shin Young-Ha
Lee Soo-Won
Yang H. J.
Yang D.-H.
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Abstract
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The purpose of this study was to purify epidermal growth factor(EGF) as growth factor from human colostrum. The effects of purified EGF fraction were directly related to the growth of cells. Results were as follows; After eliminated fat from colostrum, skim milk was obtained. We obtained the EGF fraction by performing ultrafiltration and gel filtration, and then were convicted by SDS-PAGE. The result of analysis of purified EGF fraction by high performance liquid chromatography(HPLC) was shown a peak at 31.185 min period. And it was similar with standard EGF that was shown a peak at 31.545 min. 10 ng of EGF was contained in 10 mg/mL through immunoassay to measure EGF content from isolated fraction. After SDS-PAGE, isolation degree of purified fraction was convicted through western blotting. BALB/3T3 cell was the most effectively stimulated and proliferated at 1 mg/mL concentration of the purified EGF fraction and percentage of cell proliferation was about 95%. In the case of IEC-6 cell, that was about 71%.
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KEYWORD
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epidermal growth factor, human colostrum, HPLC, immunoassay
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