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KMID : 1024620040240020164
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Food Science of Animal Resources
2004 Volume.24 No. 2 p.164 ~ p.170
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Isolation and Identification of Lactic Bacteria Containing Superior Activity of the Bile Salts Deconjugation
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Ha Chul-Gyu
Cho Jin-Kook
Chai Young-Gyu
Heo Kang-Chil
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Abstract
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The purpose of this study is to isolate probiotic lactic acid bacteria (LAB) that produced bile salts hydrolase. One hundred twenty strains were initially isolated from human feces. Based on their resistance of acid, tolerances of bile salts, and inhibitory activity against Escherichia coli, five strains were selected. A strain producing highest activity of bile salts hydrolase was identified as Lactoacillus plantarum using API carbohydrate fermentation pattern and 16S rRNA sequences, and named CK102. Lactobacillus plantarum CK102 survived at a level of 1.36108/ CFU/ in pH 2 buffer for 6 h and showed exhibited excellent bile tolerance. When L plantarum CK102 was cultured with E. coli in MRS broth, no viable cells of E. coli was detected after 18 h fermentation. These results suggest that Lactobacillus plantarum CK 102 may be commercially used for the probiotic culture.
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KEYWORD
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bile salts, lactic acid bacteria, deconjugation, isolation and identification
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