KMID : 1024620040240020171
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Food Science of Animal Resources 2004 Volume.24 No. 2 p.171 ~ p.175
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Effect of Bovine Colostral Whey Fraction containing Insulin-like Growth Factor on Cell Proliferation
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Hwang Kyung-A
Yang Hee-Jin Ha Woel-Kyu Lee Soo-Won
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Abstract
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Insulin-like growth factor-I (IGF-I) rich fraction, which was obtained molecules ranged between 30 kDa and 1 kDa, was fractionated by ultrafiltration from bovine colostral whey with 30 kDa and 1 kDa membrane. IGF-I included in fractionated IGF-I rich fraction was confirmed by SDS-PAGE and western blotting and then the quantity of IGF-I was measured by ELISA. IGF-I concentration in IGF-I rich fraction was 10ng/mg protein. Effect of IGF-I rich fraction on in vitro proliferation of several cells was tested. IEC-6 cell proliferation rate was increased 60£¥. 53£¥, 30£¥, and 20£¥ at l0ng, 1ng, 0.1ng and IGF-I of IGF-I, respectively, compared to control group which was not supplemented by IGF-I rich fraction. IGF-I rich fraction stimulated in vitro proliferation of IEC-6 cell in a dose dependent manner by increasing cell number. Detroit 551 cell proliferation was enhanced 56£¥ and 26£¥ at 10ng and 1ng level of IGF-I, respectively, compared to control group. EL-4 cell and L6 cell proliferation was increased 53£¥ and 46£¥ at 10ng of IGF-I, respectively, compared to control group.
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KEYWORD
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IGF-I, colostral whey, ultrafiltration, cell proliferation
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