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KMID : 1024620040240020182
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Food Science of Animal Resources
2004 Volume.24 No. 2 p.182 ~ p.188
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Indirect ELISA Method for Measurement of Lactoperoxidase using IgY Antibody
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Lee Seung-Bae
Choi Suk-Ho
Choi Jae-Won
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Abstract
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To determine the concentration of Lactoperoxidase (LPO), an indirect enzyme-linked immunosorbant assay(ELISA) was developed. Anti-LPO egg yolk immunoglobulin(IgY) was transferred to egg yolk by immunizing of Brown hens with LPO. The titer of purified anti-LPO IgY was 1: 520,000. The immunological response of anti- LPO IgY with -lactalbumin, -lactoglobulin, casein and lysozyme were evaluated, resulting that the anti-LPO IgY found to be a specific antibody toward LPO and no cross-reaction was observed against -lactalbumin, -lactoglobulin, casein, and lysozyme in double immunodiffusion test and ELISA test. In indirect ELISA method, coating concentration of LPO and dilution rate of anti-LPO IgY was 0.25/mL and 1:8,000 respectively. Sensitivity in the standard curve of LPO was ranged from 0.01 to 1/mL using anti-LPO IgY.
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KEYWORD
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lactoperoxidase, anti-lactoperoxidase IgY, indriect ELISA
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