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KMID : 1024620050250020203
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Food Science of Animal Resources
2005 Volume.25 No. 2 p.203 ~ p.209
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Identification of Hanwoo (Korean Native Cattle) Beef in Restaurants using Real-time PCR
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Kim Jin-Man
Nam Yong-Suk
Choi Ji-Hun
Lee Mi-Ai
Jeong Jong-Youn
Kim Cheon-Jei
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Abstract
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Real time-polymerase chain reaction (RT-PCR) is currently considered as the most sensitive method to detect low abundant DNAs in samples. Compared to conventional PCR, real-time PCR has a high reliability because of excluding false-positive results and can allow a simultaneous faster detection and quantification of target DNAs. This study was carried out to identify the Hanwoo (Korean native cattle) beef by genotyping after DNA extraction of commercial beef in 41 restaurants. Since Hanwoo, Holstein and imported cattle meat have different patterns in the MC1R gene associated with the coat colors of cattles (C-type, C/T-type or T-type), we could identify the genotype using real-time PCR The result of real-time PCR assay for beef samples in 41 restaurants which are asserted to sell Hanwoo beef only, showed that 29 of 41 samples were Hanwoo beef gene type (T-type) and 12 of 41 samples were Holstein or imported cattle gene type (C-type or C/T-type). Therefore, the proportion of Han-woo beef was and the proportion of Holstein or imported cattle meat was
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KEYWORD
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MC1R gene, real-time PCR, genotype, Hanwoo, Korean cattle
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