|
KMID : 1024620060260020263
|
|
Food Science of Animal Resources
2006 Volume.26 No. 2 p.263 ~ p.268
|
|
|
Comparison of Specific Proteins of Shiga Toxin-producing E. coli (STEC) Adhesion by Lactobacillus acidophilus Strains Using Two Dimensional Gel Electrophoresis
|
|
Kim Young-Hoon
Moon Yong-Il
|
|
|
Abstract
|
|
|
|
Probiotics including Lactobacillus acidophilus, refer to a group of nonpathogenic organisms that protect the human host against gastrointestinal(GI) infections by pathogenic bacteria such as Shiga toxin-producing E. coli(STEC). In the study, the inhibitory effects of STEC ATCC 43894 adhesion by L. acidophilus A4 was investigated on the HT-29 epithelial cells. Specific proteins regulated by cell Iysates of L. acidophilus A4 on STEC ATCC 43894 were also characterized by proteomic analysis. Both cell mass and Iysate of L. acidophilus A4 have exhibited the profound inhibitory activity on the HT-29 cells(about 1.5 log scale reduction). Two-dimensional gel electrophoresis(2-DE) revealed seven proteins that were up-regulated by cell Iysates of L. acidophilus A4 and three proteins that were down-regulated. In addition, three protein spots were only detected in the presence of cell Iysates. These results suggest that inhibitory effects of STEC adhesion by L. acidophilus may be due to the regulation of specific protein of STEC.
|
|
|
KEYWORD
|
|
|
STEC adhesion, Lactobacillus acidophilus, cell Iysate, two dimensional gel electrophoresis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|