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KMID : 1024620080280030306
Food Science of Animal Resources
2008 Volume.28 No. 3 p.306 ~ p.311
Reduction of the Antigenicity of Powdered Milk by Gamma Irradiation
Shin Ji-Hye

Jeong Seok-Geun
Han Gi-Sung
Jang Ae-Ra
Chae Hyun-Seok
Yoo Young-Mo
Ahn Chong-Nam
Lee Ju-Woon
Jo Cheorun
Lee Wan-Kyu
Ham Jun-Sang
Abstract
The aim of this work was to examine the reduction in antigenicity of milk proteins in powdered milk by gamma irradiation which is increasingly used for food safety. Skim milk powder samples were exposed to irradiation doses of 1, 5, and 10 kGy. A greater reduction of -casein and -casein was found than -casein and -casein by capillary electrophoresis. Competitive indirect ELISA and passive cutaneous anaphylaxis tests using guinea pigs showed a reduction in antigenicity of powdered milk by 10kGy gamma irradiation. These results indicated that gamma irradiation reduce allergenicity of milk proteins by structural changes of -casein and -casein, and can be useful for dairy products.
KEYWORD
Powder milk, gamma irradiation, antigenicity
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