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KMID : 1024620140340050665
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Food Science of Animal Resources
2014 Volume.34 No. 5 p.665 ~ p.673
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Comparison of Culture, Conventional and Real-time PCR Methods for Listeria monocytogenes in Foods
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Kim Dong-Hyeon
Chon Jung-Whan
Kim Hyun-Sook
Kim Hong-Seok
Choi Da-som
Kim Young-Ji
Yim Jin-Hyeok
Moon Jin-San
Seo Kun-Ho
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Abstract
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We compared standard culture methods as well as conventional PCR and real-time PCR for the detection of Listeria monocytogenes (L. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. No statistical differences were observed in sensitivity between the two selective media in all foods. In total, real-time PCR assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compared with standard culture methods. Conventional culture methods showed poor performance in detecting L. monocytogenes in food with high levels of background microflora, generating numerous false negative results. While the detection of L. monocytogenes in fresh cut vegetable by culture methods was hindered only by L. innocua, various background microflora, such as L. innocua, L. welshimeri, L. grayi, and Enterococcus faecalis appeared on the two selective media as presumptive positive colonies in raw beef indicating the necessity of improvement of current selective media. It appears that real-time PCR is an effective and sensitive presumptive screening tool for L. monocytogenes in various types of foods, especially foods samples with high levels of background microflora, thus complementing standard culture methodologies.
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KEYWORD
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Listeria monocytogenes, culture method, profiling of false-positive colonies, conventional PCR, real-time PCR
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