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KMID : 1024620160360060752
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Food Science of Animal Resources
2016 Volume.36 No. 6 p.752 ~ p.759
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Mathematical Model for Predicting the Growth Probability of Staphylococcus aureus in Combinations of NaCl and NaNO2 under Aerobic or Evacuated Storage Conditions
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Lee Jee-Yeon
Gwak Eun-Ji
Ha Ji-Myeong
Kim Se-Jeong
Lee Soo-Min
Lee Hee-Young
Oh Mi-Hwa
Park Beom-Young
Oh Nam-Su
Choi Kyoung-Hee
Yoon Yo-Han
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Abstract
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The objective of this study was to describe the growth patterns of Staphylococcus aureus in combinations of NaCl and NaNO2, using a probabilistic model. A mixture of S. aureus strains (NCCP10826, ATCC13565, ATCC14458, ATCC23235, and ATCC27664) was inoculated into nutrient broth plus NaCl (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75%) and NaNO2 (0, 15, 30, 45, 60, 75, 90, 105, and 120 ppm). The samples were then incubated at 4, 7, 10, 12 and 15°C for up to 60 d under aerobic or vacuum conditions. Growth responses [growth (1) or no growth (0)] were then determined every 24 h by turbidity, and analyzed to select significant parameters (p<0.05) by a stepwise selection method, resulting in a probabilistic model. The developed models were then validated with observed growth responses. S. aureus growth was observed only under aerobic storage at 10-15°C. At 10-15°C, NaCl and NaNO2 did not inhibit S. aureus growth at less than 1.25% NaCl. Concentration dependency was observed for NaCl at more than 1.25%, but not for NaNO2. The concordance percentage between observed and predicted growth data was approximately 93.86%. This result indicates that S. aureus growth can be inhibited in vacuum packaging and even aerobic storage below 10°C. Furthermore, NaNO does not effectively inhibit S. aureus growth.
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KEYWORD
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predictive model , S. aureus , NaCl , NaNO2 , processed meat products
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