|
KMID : 1024620180380051008
|
|
Food Science of Animal Resources
2018 Volume.38 No. 5 p.1008 ~ p.1018
|
|
|
Isolation and Characterization of an Anti-listerial Bacteriocin from Leuconostoc lactis SD501
|
|
Hwang In-Chan
Oh Ju-Kyoung
Kim Sang-Hoon
Oh Se-Jong
Kang Dae-Kyung
|
|
|
Abstract
|
|
|
|
Although bacteriocins with anti-listerial activity have been isolated from a wide variety of lactic acid bacteria, little is known about those from Leuconostoc lactis, a heterofermentative bacterium that produces diacetyl and exopolysaccharides in dairy foods. In this study, an anti-listerial bacteriocin was isolated from Leuc. lactis SD501 and characterized. It was particularly potent against Listeria monocytogenes and also inhibited Enterococcus faecalis. Anti-listerial activity reached a maximum during the early stationary phase and then decreased gradually. The anti-listerial substance was sensitive to proteinase K and α-chymotrypsin, confirming its proteinaceous nature. Its activity remained stable at pH values ranging from 1 to 10. In addition, it was strongly resistant to high temperatures, retaining its activity even after incubation for 15 min at 21℃. The apparent molecular mass of the partially purified anti-listerial bacteriocin was approximately 7 kDa. The characteristics of the SD501 bacteriocin, including its small molecular size (<10 kDa), strong anti-listerial activity, wide pH stability and good thermostability, indicate its classification as a Class IIa bacteriocin.
|
|
|
KEYWORD
|
|
|
Leuconostoc lactis , bacteriocin , Listeria monocytogenes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|