KMID : 1024620210410040608
|
|
Food Science of Animal Resources 2021 Volume.41 No. 4 p.608 ~ p.622
|
|
Enzymatic Hydrolysis of Ovotransferrin and the Functional Properties of Its Hydrolysates
|
|
Rathnapala Ethige Chathura Nishshanka
Ahn Dong-Uk Abeyrathne Edirisingha Dewage Nalaka Sandun
|
|
Abstract
|
|
|
Bioactive peptides have great potentials as nutraceutical and pharmaceutical agents that can improve human health. The objectives of this research were to produce functional peptides from ovotransferrin, a major egg white protein, using single enzyme treatments, and to analyze the properties of the hydrolysates produced. Lyophilized ovotransferrin was dissolved in distilled water at 20 mg/mL, treated with protease, elastase, papain, trypsin, or ¥á-chymotrypsin at 1% (w/v) level of substrate, and incubated for 0?24 h at the optimal temperature of each enzyme (protease 55¡É, papain 37¡É, elastase 25¡É, trypsin 37¡É, ¥á-chymotrypsin 37¡É). The hydrolysates were tested for antioxidant, metal-chelating, and antimicrobial activities. Protease, papain, trypsin, and ¥á-chymotrypsin hydrolyzed ovotransferrin relatively well after 3 h of incubation, but it took 24 h with elastase to reach a similar degree of hydrolysis. The hydrolysates obtained after 3 h of incubation with protease, papain, trypsin, ¥á-chymotrypsin, and after 24 h with elastase were selected as the best products to analyze their functional properties. None of the hydrolysates exhibited antioxidant properties in the oil emulsion nor antimicrobial property at 20 mg/mL concentration. However, ovotransferrin with ¥á-chymotrypsin and with elastase had higher Fe3+-chelating activities (1.06¡¾0.88%, 1.25¡¾0.24%) than the native ovotransferrin (0.46¡¾0.60%). Overall, the results indicated that the single-enzyme treatments of ovotransferrin were not effective to produce peptides with antioxidant, antimicrobial, or Fe3+-chelating activity. Further research on the effects of enzyme combinations may be needed.
|
|
KEYWORD
|
|
bioactive peptides, enzyme hydrolysis, functional properties, hydrolysates, ovotransferrin
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|