|
KMID : 1025520000420030371
|
|
Journal of Animal Science and Technology
2000 Volume.42 No. 3 p.371 ~ p.378
|
|
|
Production of Specific Egg Yolk Antibody against K99(F5) Fimbriae from Enterotoxigenic Escherichia coli
|
|
|
|
|
Abstract
|
|
|
|
The adhesive antigen K99 of Enterotoxigenic Escherichia coil strain was isolated and purified. The K99 antigen consist of single peptide with molecular mass of 18kDa and was demonstrated by Western blot analysis. In addition, the antigen-antibody reaction of K99 antigen was specifically occurred only with K99 monoclonal antibody. The antibody titer of immunized hen with adhesive antigen was shown at 2 weeks after immunization. At the time of 8 weeks, the titer of egg yolk revealed higher than that of sera. And-K99 yolk antibody did not react with different strains(K88, 987P) but reacted only with ETEC K99 strains. The immunoglobulin(IgY) content in an egg yolk and in water soluble fraction were 150㎎ and 120㎎, respectively. Thus, the recovery rate of IgY was 87 percent in this study.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|