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KMID : 1025520000420040433
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Journal of Animal Science and Technology
2000 Volume.42 No. 4 p.433 ~ p.442
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Characteristics of α s2-casein Gene Using PCR Technique in Korean Native Goat
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Abstract
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This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from the analysis of genetic characteristics of α_(S2-) casein gene in Korean Native goat using PCR-RFLP. This study confirmed the amplified product of 1.3kb fragment obtained from the amplification of a α_(S2-)casein loci by PCR.
The frequency of a α_(S2-)casein genotype in Korean Native goat was 100% for AA type and the frequencies of a α_(S2-)casein genotype in Saanen were 80 and 20% for AA and AB genotypes. The frequency of a α_(S2-)casein A allele was 1.000 in Korean Native goat, and the frequencies of a α_(S2-)casein A and B alleles were 0.900 and 0.100 in Saanen, respectively.
The nucleotide sequence of a α_(S2-)casein gene of Korean Native goat was 99.0% homology with 12 nucleotide sequences difference of that of goat reported in GeneBank(AJ238965.1, AJ238967.1) and was 95.0% homology with 57 nucleotide sequences difference of that of Holstein reported in Gene Bank(M94327).
Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphisms and sequences for a α_(S2-)casein gene should be used as basic data for preservation and improvement of genetic resources in Korean Native goat breeding.
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KEYWORD
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