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KMID : 1025520010430010111
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Journal of Animal Science and Technology
2001 Volume.43 No. 1 p.111 ~ p.120
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Determination of Vitamin D2 and D3 in Milk using HPLC
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백승천/Baick, S. C.
김철현/정운현/하영식/Kim, C. H./Chung, W. H./Ha, Y. S.
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Abstract
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This study was carried out to compare three HPLC column and various mobile phase for the determination of vitamin D₂and D₃in milk. The saponified samples were extracted with hexane, and the extracts containing interfering substances were further clean up by using the Sep-pak cartridge containing silica.
The Symmetry C_(18) column provided more satisfactory analysis data than the other columns in terms of precision and selectivity. The Symmetry C_(18) column showed a linear relationship between the concentration increments and peak areas of in vitamin D₂and D₃in the standard solution(r=0.998∼999, P$lt;0.01). The recovery percent(97.10∼99.78%) of vitamin D₃ in the samples saponified and extracted were much higher than those(51.90∼80.96%) in the samples only clean-up treated.(51.90∼80.96%).
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KEYWORD
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