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KMID : 1025520080500030293
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Journal of Animal Science and Technology
2008 Volume.50 No. 3 p.293 ~ p.300
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Expression Profiles of Triacylglycerol Biosynthesis Genes on Fattening Stages in Hanwoo
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Kim Nam-Kuk
Kim Sung-Kon
Heo Kang-Nyeong
Yoon Du-Hak
Lee Chang-Soo
Im Seok-Ki
Park Eung-Woo
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Abstract
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Muscle fat content including intramuscular fat content(IMF) is the most important parameter in meat quality of cattle. Triacylglycerol is the major component of fat and is found in adipocyte and skeletal muscle. In present study, we carried out the determined of triacylglycerol contents and mRNA expression patterns of genes related with triacylglycerol biosynthesis such as ACSS2, GPAT, MGAT and DGAT in Hanwoo longissimus muscle using the real-time PCR. The triacylglycerol contents were continuously increased during the fattening stages. Interestingly, the contents of triacylglycerol were 7.4 fold higher(0.34 vs. 2.51, P<0.01) in 27 months old group than 12 months old group. The mRNA levels of ACSS2, GPAT and DGAT genes were also increased during fattening stages, whereas that of MGAT gene did not show difference among the stages. Thus, these results suggested that increasing of the triacylglycerol contents in longissimus muscle during fattening stages may be related with increased expressions of triacylglycerol biosynthesis genes(ACSS2, GPAT and DGAT). These results will be helpful to understand the mechanism of muscle fat deposition in skeletal muscle.
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KEYWORD
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Triacylglycerol, Real-time PCR, Fattening, Hanwoo
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