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KMID : 1025520120540020133
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Journal of Animal Science and Technology
2012 Volume.54 No. 2 p.133 ~ p.139
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Characteristics of α-Amylase and Protease Produced from Bacillus amyloliquefacies CNL-90 Isolated from Malt Grain
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Bae Hyoung-Churl
Choi Seong-Hyun
Na Seuk-Han
Nam Myoung-Soo
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Abstract
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A bacterium, identified as Bacillus amyloliquefacies CNL-90 using 16S rDNA analysis, was isolated from malt grain. The optimal activities of its α-amylase and protease were observed at pH 6 and 60℃, and at pH 6 and 50C, respectively although their activities remained stable at pH 7 and 40℃ for α-amylase and at pH 7 and 50℃ for protease. After solid-state fermentation of B. amyloliquefacies CNL-90 on wheat bran for 72hr or 144hr, the α-amylase and protease activities were 170,000 and 290,000 units/kg, and 290,000 and 310,000 units/kg, respectively. The viable bacterial cell counts were 1.5×109 CFU/g and 2.2 × 109 CFU/g at 72hr and 144hr of the solid-state fermentation, respectively. A feeding trial with a total of 127 piglets was also conducted. The animals were divided into two groups: an experimental group fed with the fermented product(63 piglets) and a control group(64 piglets). The growth rate of the experimental group was 6.66% higher than that of the control group(P<0.05). The results of this study indicate that the α-amylase and protease from B. amyloliquefacies CNL-90 can be used for industrial applications due to their activity in production of carbohydrate hydrolysates.
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KEYWORD
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Bacillus amyloliquefacies CNL-90, α-amylase, Protease, Growth rate
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