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KMID : 1033620140410030132
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Clinical and Experimental Reproductive Medicine
2014 Volume.41 No. 3 p.132 ~ p.136
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Observation of sperm-head vacuoles and sperm morphology under light microscope
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Park Yong-Seog
Park Sol
Ko Duck-Sung
Park Dong-Wook
Seo Ju-Tae
Yang Kwang-Moon
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Abstract
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Objective:The presence of sperm-head vacuoles has been suspected to be deleterious to the outcomes of assisted reproductive technology (ART). It is difficult to accurately distinguish morphologically abnormal sperm with vacuoles under a light microscope. This study was performed to analyze the result of the observation of sperm-head vacuoles using Papanicolaou staining under a light microscope and whether the male partner’s age affects these vacuoles.
Methods:Sperm morphology with vacuoles was evaluated using Papanicolaou staining and observed under a light microscope (400×) in 980 men. The normal morphology was divided into three categories (group A, <4% of normal morphology; group B, 4%-14% of normal morphology; and group C, >14% of normal morphology). The criteria for the sperm-head vacuoles were those given in the World Health Organization manual. For the analysis of the age factor, the participants were divided into the following groups: 26-30 years, 31-35 years, 36-40 years, 41-45 years, and 46-50 years.
Results:The percentage of sperm-head vacuoles increased with normal sperm morphology (group A vs. groups B, C) (p<0.05). In the case of the age factor, a statistically significant difference was not observed across any of the age groups.
Conclusion: A majority of the sperm-head vacuoles showed a statistically significant difference among normal morphology groups. Therefore, we should consider the probability of the percentage of sperm-head vacuoles not increasing with age but with abnormal sperm morphology. A further study is required to clarify the effect of the sperm-head vacuoles on ART outcomes.
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KEYWORD
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Papanicolaou staining, Semen analysis, Sperm-head vacuole, Sperm morphology
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