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KMID : 1033620170440010052
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Clinical and Experimental Reproductive Medicine
2017 Volume.44 No. 1 p.52 ~ p.55
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A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser
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Chen Huan Hua
Feng Gui Xue
Zhang Bo
Zhou Hong
Shu Jin Hui
Gan Xian You
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Abstract
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The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.
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KEYWORD
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Cryopreservation, Immotile spermatozoa, Intracytoplasmic sperm injections, Lasers
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