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KMID : 1033620220490030196
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Clinical and Experimental Reproductive Medicine
2022 Volume.49 No. 3 p.196 ~ p.201
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Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation
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Kim Seul-Ki
Paik Hae-Rin
Lee Jung-Ryeol
Jee Byung-Chul
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Abstract
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Objective: This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer.
Methods: Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within- and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance.
Results: The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45?0.84) and semen volume (ICC, 0.67; 95% CI, 0.45?0.84).
Conclusion: Our findings showed that repeated ejaculates from patients with cancer had stable SDF levels.
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KEYWORD
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Cryopreservation, DNA fragmentation, Fertility preservation, Semen analysis, Sperm
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