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KMID : 1034820190150040469
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Molecular & Cellular Toxicology
2019 Volume.15 No. 4 p.469 ~ p.475
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Nitrendipine, an antihypertensive alpha calcium channel blocker, is cytotoxic to neuroblastoma cells
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Rivera Isabel
Cauvi David M.
Arispe Nelson
De Maio Antonio
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Abstract
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Backgrounds: Alpha calcium channel blockers (CCB) are widely prescribed for the treatment of hypertension, including Nitrendipine (NTD). The anti-hypertension effect of NTD is related to binding and blocking the activity of the voltage-gated L-type calcium channels. These channels consist of four subunits (α1, α2/δ, γ and β subunits), localized in the plasma membrane.
Methods: Cellular proliferation was determined by the XTT method and apoptosis was measured by flow cytometry. The expression of the α1c subunit (Cacna1c) of the voltage-gated L-type calcium channels was reduced by siRNA and assessed by quantitative RT-PCR.
Results: NTD blocked the proliferation of murine neuroblastoma (N2a cells) and induced apoptosis in a concentration-dependent manner. A decrease in Cacna1c expression did not have a significant effect on NTD toxicity of N2a cells.
Conclusion: NTD induced apoptosis on neuroblastoma cells, appears to be independent of the expression of the L-type calcium channel.
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KEYWORD
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Alpha channel calcium blockers, Hypertension, Nitrendipine, Voltage-gated L-type calcium channels
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