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KMID : 1034820200160020149
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Molecular & Cellular Toxicology
2020 Volume.16 No. 2 p.149 ~ p.158
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MiRNA-338-5p reduced inflammation through TXNIP/NLRP3 inflammasome axis by CXCR4 in DSS-induced colitis
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Huang Xunru
Lin Yijuan
Zheng Xueyan
Wang Chengdang
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Abstract
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Backgrounds: miRNAs, about 20?25 bases in length, are short-chain non-coding RNAs existing in the body, which are widely involved in the biological regulation of the organism. miRNAs inhibit the expression of target genes by specifically binding to target genes, thereby exerting biological effects.
Methods: We investigated the function of miRNA-338-5p on inflammation and its possible mechanisms in colitis
Results: In DSS-induced colitis model, we found that miRNA-338-5p expression was reduced. Therefore, down-regulation of miRNA-338-5p increased inflammation and induced CXCR4, TXNIP, and NLRP3 protein expression in in vitro model. Meanwhile, over-expression of miRNA-338-5p reduced inflammation and suppressed CXCR4, TXNIP, and NLRP3 protein expression in in vitro model. Therefore, miRNA-338-5p may possess anti-inflammatory effects in colitis. However, si-CXCR4 reduced the anti-inflammatory effects of miRNA-338-5p in invitro model. Then, si-NLRP3 also reduced the anti-inflammatory effects of miRNA-338-5p in in vitro model. These results showed that miRNA-338-5p reduced inflammation in colitis through the TXNIP/NLRP3 inflammasome axis by CXCR4.
Conclusion: MiRNA-338-5p may potentially serve as novel therapeutic avenues for colitis.
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KEYWORD
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miRNA-338-5p, TXNIP, NLRP3, CXCR4, Colitis
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