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KMID : 1034820200160030301
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Molecular & Cellular Toxicology
2020 Volume.16 No. 3 p.301 ~ p.309
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Deciphering the possible role of H2O2 in methylmercury-induced neurotoxicity in Xenopus laevis
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Fu Xufeng
Yang Xiuyu
Du Xing
Cui Qinghua
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Abstract
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Backgrounds: Methylmercury (MeHg) is regarded as a developmental neurotoxicant but the detailed mechanism remains not completely clear.
Methods: The Xenopus laevis embryos were exposed to methylmercury chloride and the expression of neurodevelopment and oxidative stress genes was detected by qRT-PCR or Western blotting. PC12 cells were exposed to various levels of H2O2, and then cell cycle, neurite length, neurodevelopment-related genes, protein expression of apoptosis and autophagy were detected.
Results: The genes of neurodevelopment and oxidative stress were disrupted by methylmercury chloride and H2O2 were increased interestingly in X. laevis embryos. Then, PC12 cells were exposed to H2O2 and the results showed the cell cycle, neurite length, and neurodevelopment-related genes, the proteins apoptosis and autophagy were changed.
Conclusion: These results supported the idea that neurodevelopment-related gene expression was regulated by oxidative stress and that apoptosis and autophagy pathways were activated by H2O2 and involved in methylmercury neurotoxicity.
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KEYWORD
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Methylmercury, Neurotoxicity, H2O2, Oxidative stress, Apoptosis, Autophagy
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