KMID : 1034820200160040441
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Molecular & Cellular Toxicology 2020 Volume.16 No. 4 p.441 ~ p.453
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Curcumin promotes oxidative stress, apoptosis and autophagy in H9c2 rat cardiomyoblasts
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Zepeda-Quiroz Ivan
Sanchez-Barrera Helen Colin-Val Zaira Robledo-Cadena Diana Xochiquetzal Rodriguez-Enriquez Sara Lopez-Marure Rebeca
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Abstract
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Background: Curcumin, a polyphenol derived from Curcuma longa, has some adverse effects on heart; however, its toxic effects on cardiac cells are poorly understood.
Objective: To evaluate the toxicity of curcumin on H9c2 rat cardiomyoblasts. To this, H9c2 cells were exposed to different concentrations of curcumin and proliferation, viability, cell cycle, oxidative stress, mitochondrial membrane potential (¥Ä¥×m), death and autophagy were evaluated.
Results: Curcumin caused concentration-dependent inhibition of H9c2 cells proliferation and viability. A higher sub-G1 population was observed in cells treated with curcumin, which was related with phosphatidylserine translocation and increase of activated caspase-9, indicating apoptotic death. Curcumin induced oxidative stress and decreased ¥Ä¥×m causing mitochondrial dysfunction. Additionally, it promoted autophagy, revealed by higher LC3B and beclin-1 protein expression and mitophagy.
Conclusion: Curcumin exhibited toxic effects in cardiac cells and further studies are required to validate its therapeutic potential and use as anti-inflammatory and anti-oxidant agent in the cardiovascular system.
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KEYWORD
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Curcumin, Cardiomyoblasts, Cell cycle, Oxidative stress, Cell death, Mitochondrial damage, Autophagy, Mitophagy
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