KMID : 1039220190290030414
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Journal of Korean Society of Occupational and Environmental Hygiene 2019 Volume.29 No. 3 p.414 ~ p.419
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Effect of Non-thermal Dielectric Barrier Discharge Plasma by Air Volume against Mycobacterium Tuberculosis
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Son Eun-Soon
Kim Yong-Hee Paik Nam-Won Lee Il-Young Kim Eun-Hwa Park Hae-Ryoung Lee Jong-Seok
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Abstract
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Objectives: The objective of this study was to evaluate the inhibitory effect of non-thermal dielectric barrier discharge (DBD) plasma by air volume against Mycobacterium tuberculosis (MTB).
Methods: Plasma generators (TB-300, Shinyoung Airtec, Seongnam-si, Korea) were operated in a 2A type biosafety cabinet. The plasma generator was set to a wind flow rate of 14 (80 m3/h), 18 (110 m3/h), and 22 (150 m3/h), and exposure times were set to 0 hours, 3 hours, 6 hours, 9 hours, and 24 hours.
Results: The inhibitory effects of plasma at air volume 14 with prolonged exposure time of three hours was 20%, 64% at six hours, 82.3% at nine hours, and 100% after 24 hours exposure. With air volume of 18, the inhibitory effects upon plasma exposure were 36% for three hours, and 100% from 24 hours. Greater air volume resulted in greater inhibition of tuberculosis bacterial growth. In particular, the maximum inhibitory effect (100%) was shown in air volume of 22 (150 m3/h) after three hours of plasma exposure.
Conclusions: The results showed the correlating inhibitory effects of plasma on the growth of MTB in combination with increasing plasma exposure time and air volume.
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KEYWORD
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Mycobacterium tuberculosis, plasma, Non-thermal dielectric barrier discharge
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