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KMID : 1039220210310040473
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Journal of Korean Society of Occupational and Environmental Hygiene
2021 Volume.31 No. 4 p.473 ~ p.483
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Acute Toxicity Study of the 2-butoxyethanol by Intratracheal Instillation in Male Sprague-Dawley Rats
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Kim Hyeon-Young
Kim In-Hyeon
Kim Min-Seok
Kim Sung-Hwan
Lee Kyu-Hong
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Abstract
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Objectives: The present study aimed to evaluate the potential toxicity of 2-butoxyethanol after intratracheal instillation in male rats.
Methods: In order to calculate median lethal dose (LD50) of 2-butoxyethanol using Probit analysis with SAS program, the 2-butoxyethanol was administered with dose levels of 0, 101.64, 203.28 and 406.56 mg/kg by once intratracheal instillation to male rats. During the test period, clinical signs, mortality, body weights, organ weights, hematology, and serum biochemistry were examined. At the end of 14 days observation period, all animals were sacrificed and gross finding and histopathological examination were performed.
Results: All animals of 406.56 mg/kg group died within 2 weeks after the administration of 2-butoxyethanol. Treatment-related clinical signs, gross observation and histopathological changes (mucous cell hyperplasia, alveolar macrophage aggregation, and hemorrhage) of lung exhibited an increased in 2-butoxyethanol treated groups in a dose dependent manner. However, there were no changes in the organ weights, hematology and serum biochemistry, and histopathology of any other organ except lung.
Conclusions: On the basis of the results, it was concluded that a single intratracheal instillation of 2-butoxyethanol in male Sprague-Dawley rats resulted in some adverse effects on mortality, clinical sign, and histopathology in the lung. In the experimental conditions, the LD50 of 2-butoxyethanol was considered to be 287.2 mg/kg and lung was founded to be the target organ of 2-butoxyethanol.
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KEYWORD
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acute toxicity, 2-butoxyethanol, intratracheal instillation, LD50, pulmonary toxicity
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