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KMID : 1094719960010010018
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Biotechnology and Bioprocess Engineering
1996 Volume.1 No. 1 p.18 ~ p.18
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Continuous production of fructooligosaccharides using fructosyltransferase immobilized on ion exchange resin
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Yun Jong-Won
Song Seung-Koo
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Abstract
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A continuous production of fructooligosaccharides from sucrose was investigated by fructosyltransferase immobilized on a high porous resin, Diaion HPA 25. The optimum pH (5.5) and temperature (55°C) of the enzyme for activity was unaltered by immobilization, and the immobilized enzyme became less sensitive to the pH change. The optimal operation conditions of the immobilized enzyme column for maximizing the productivity were as follows: 600 g/L of sucrose feed concentration, flow rate of superficial space velocity 2.7 h?1. When the enzyme column was run at 50°C, about 8% loss of the initial activity of immobilized enzyme was observed after 30 days of continuous operation, during which high productivity of 1174 g/L·h was achieved. The kinds of products obtained using the immobilized enzyme were almost the same as those using soluble enzymes or free cells.
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KEYWORD
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Fructooligosaccharides, Fructosyltransferase, Immobilized enzyme, Immobilized cells
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