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KMID : 1094719970020010062
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 1 p.62 ~ p.63
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Simple DNA elution from agarose gels
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Gal Sang-Wan
Cho Yong-Un
Kim Hong-Chul
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Abstract
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We developed a simple DNA elution method from agarose gels. After electrophoresis of DNA in an agarose gel, the DNA fragment to be recorved was excised out of gel with a scalpel. The excised gel was placed in the middle of small Parafilm piece, and the Parafilm was folded over the gel piece. Using the petriplate, or thumb, the gel piece was pressed between the Parafilm. Upon squeezing, the DNA inside of the gel gets extruded along with the buffer. The droplets were collected with a pipet. The DNA was then purified by conventional phenol: chloroform extraction method. Typical yields are greater than 50% as determined by UV absorbance.
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KEYWORD
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parafilm, squeezing, DNA elution
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