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KMID : 1094719970020020113
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 2 p.113 ~ p.116
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Separation and purification of useful proteins using hydrogel ultrafiltration
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Park Chang-Ho
Son Chang-Kyu
Park Jong-Hwa
Chung In-Sik
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Abstract
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The hydrogel process is a different form of ultrafiltration and has been used to separate biological molecules. In this study, the gel pore size was predicted by pulse NMR technique and neural network using a data base obtained from gel filtration chromatography and diffusion experiment. Recombinant alkaline phosphatase expressed in insect cells was concentrated 1.5 times by hydrogel ultrafiltration by swelling at 20°C and collapsing at 35°C at 53?65% separation efficiency and 78?83% enzyme recovery. Wild and recombinantAutographa californica nuclear polyhedrosis viruses (AcNPV) were also concentrated 1.4 and 1.6 times of the feed solution at 48.5 and 60.0% separation efficiency, respectively. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of AcNPV and recombinant proteins from insect cells.
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KEYWORD
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NMR, neural network, Autographa californica nuclear polyhedrosis viruses, insect cells, recombinant alkaline phosphatase
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