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KMID : 1094719980030020061
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Biotechnology and Bioprocess Engineering
1998 Volume.3 No. 2 p.61 ~ p.61
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Purification and structural characterization of glycolipid biosurfactants fromPseudomonas aeruginosa YPJ-80
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Park Oh-Jin
Lee Young-Eun
Cho Joong-Hoon
Shin Hyun-Jae
Yoon Byung-Dae
Yang Ji-Won
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Abstract
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Glycolipids produced byPseudomonas aeruginosa YPJ-80 were characterized by chromatographic and spectroscopic techniques as a mixture of two rhamnolipids. For recovery of glycolipids from the culture broth, various isolation methods including ultrafiltration, adsorption and solvent extraction were compared. Ultrafiltration showed the best results in terms of glycolipids recovery. Further purification for spectroscopic analysis was carried out by adsorption chromatography and preparation thin layer chromatography. From the spectroscopic analysis, such as IR spectroscopy, FAB-MS,1H-NMR and13C-NMR and hydrolysis analysis, the glycolipids were identified as L-α-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate and 2-O-α-L-rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate. Monorhamnolipid and dirhamnolipid lowered the surface tension of water to 28.1 mN/m and 29.3 mN/m, respectively.
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KEYWORD
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Pseudomonas, glycolipid, biosurfactant, emulsification
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