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KMID : 1094719990040010063
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 1 p.63 ~ p.65
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Clearance of false-positive antigen-antibody reactions of a diagnostic antigen produced inEscherichia coli with human sera
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Noh Kap-Soo
Kim Jong-Wan
Ha Suk-Hoon
Yoo Wang-Don
Jeon Yeong-Joong
Kim Hyun-Su
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Abstract
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Although many pharmaceutically useful proteins are produced inE. coli expression system, it is very rare for the system to be used in the production of diagnostic antigen due to a major problem,i.e., false-positive reaction ofE. coli host-derived proteins contaminating purified diagnostic antigen with human sera. The N (nucleocapsid) protein of Seoul virus causing haemorrhagic fever with renal syndrome (HFRS) was produced inE. coli BL21 (DE3), and used for the detection of N protein-specific antibodies in human sera. Using the N protein as a diagnostic antigen of HFRS, the false-positive reaction was cleared by merely mixing the test sera with the extract ofE. coli host strain not harboring expression plasmid.
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KEYWORD
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diagnostic antigen, E. coli, false-positive reaction
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