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KMID : 1094719990040020119
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 2 p.119 ~ p.119
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Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng
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Son Sung-Ho
Choi Sung-Mee
Hyung Soo-Jin
Yun Seung-Rho
Choi Myung-Suk
Shin Eun-Myoung
Hong Yong-Pyo
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Abstract
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Among the various plant growth regulators (PGR), 9.0 μM 2,4-D(2,4-dichlorophenoxyacetic acid) turned out to be the best for callogenesis and 4.5 μM 2,4-D was revealed to be more suitable for callus proliferation. Of the four basal media, the highest number of 8.2 adventitious roots was induced in Woody plant medium (WPM) containing 14.8 μM IBA(indole 3-butyic acid). F tests on each auxin treatment in the ANOVA revealed highly significant difference at α=0.01. The number of multiple adventitious roots (MARs) formed in each adventitious roots highly depended on the types and levels of auxin tested. At both 24.6 μM and 34.4 μM IBA, more than 25 MARs were induced after 4 weeks of culture. When different medium were applied, immersion type showed the highest fresh weight of MARs at the time of harvest. With 160 g fresh weight of inoculum, about 14.2 times of biomass was obtained after 6 weeks of culture. Identical fractionation pattern of the amplified fragment length polymorphism (AFLP) analysis was observed between the adventitious root and donor of a mountain ginseng. This observation suggested that any rearrangements of the genomic DNA had not occurred in the adventitious roots after 1 year of cultures. The cultivated ginseng and the mountain ginseng groups could be distinguished by 2 AFLP markers.
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KEYWORD
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mountain ginseng, mass production, adventitious root, bioreactor, AFLP analysis
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