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KMID : 1094719990040040253
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 4 p.253 ~ p.253
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Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions
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Sharp Janet M.
Doran Pauline M.
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Abstract
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Hairy roots and suspended cells of transformedNicotiana tabacum were used to produce full length murine IgG1 monoclonal antibody. The maximum amount of antibody accumulated per g dry weight in the hairy root cultures was 6.5 times that in the suspension cultures. Up to 48% of the antibody in the suspension cultures was found extracellularly, while a maximum of only 17% was recovered from the hairy root medium. The amount of assembled antibody in the root and suspension cultures was significantly reduced by intracellular and/or extracellular antibody degradation soon after the end of the exponential growth phase. Bacitracin, a polypeptide antibiotic, has been shown in previous work to prevent degradation of peptides and hormones in plant and mammalian systems. Treatment of hairy roots and cell suspensions with 100 μg/mL bacitracin was not sufficient to prevent loss of antibody from the cultures, but improved the specific growth rates by up to 53%. At concentrations of 250 μg/mL and above, bacitracin had a toxic effect on hairy roots, which may limit the application of this peptide in plant tissue culture.
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KEYWORD
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antibody stability, bacitracin, hairy roots, monoclonal antibody, suspended plant cells
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