|
KMID : 1094719990040040264
|
|
Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 4 p.264 ~ p.264
|
|
|
Ultrasonic cell separator as a cell retaining device for high density cultures of plant cell
|
|
Shin Myoung-Keun
Park Kyung-Moon
Cho Gyu-Heon
|
|
|
Abstract
|
|
|
|
A system of ultrasonic filter device consisted of an ultrasonic generator, ultrasonic cell separation chamber (resonator) and a guide column, which was developed for suspension cultures of a plant cell. The key operation parameters affecting the efficiency of separation of cells from medium fluid were found to be the voltage of ultrasonic generator, the convective flow rate, and the distance between transducer and reflector. In the high density cultures ofAloe saponaria (>17 g DCW/L), the ultrasonic filter was so efficient that the cell holding time in the separation chamber was 10-fold higher than the case without ultrasonic wave at a convective flow rate of 0.24 cm/min. Furthermore, in perfusion type of high cell density cultures, cell aggregates were observed to be densely held in the ultrasonic chamber by ultrasonic force overcoming both gravitational and drag forces by pump. The accumulated cells were finally overflowed after the holding capacity of the chamber was reached. Back pressure was applied periodically to the resonator to flush cells back to bioreactor. The ultrasonic cell separator could operate over 75 min at a convective flow rate of 0.1 cm/min and at a cell concentration of 17 g DCW/L.
|
|
|
KEYWORD
|
|
|
ultrasound, perfusion system, plant cell cultures, cell separator, Aloe saponaria Haw
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|