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KMID : 1094720000050010017
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 1 p.17 ~ p.22
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Chiral separation of tryptophan enantiomers by liquid chromatography with BSA-silica stationary phase
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Kim Kwon-Il
Lee Ki-Say
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Abstract
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The separation of tryptophan enantiomers was carried out with medium-pressure liquid chromatography using BSA (bovine serum albumin)-bonded silica as a chiral stationary phase. The influence of various experimental factors such as pH and ionic strength of mobile phase, separation temperature, and the presence of organic additives on the resolution was studied. In order to expand this system to preparative scale, the loadability of sample and the stability of stationary phase for repeated use were also examined. The separation of tryptophan enantiomers was successful with this system. The data indicated that a higher separation factor (α) was obtained at a higher pH and lower temperature and ionic strength in mobile phase. Addition of organic additives (acetonitrile and 2-propanol) in mobile phase contributed to reduce the retention time of L-tryptophan. About 30% of the separation factor was reduced after 80 days of repeated use.
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KEYWORD
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BSA-silica, chiral separation, chiral stationary phase, liquid chromatography, tryptophan enantiomers
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