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KMID : 1094720000050010032
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 1 p.32 ~ p.32
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Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol
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Chandrakant Priya
Bisaria Virendra S.
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Abstract
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Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of glucose-xylose mixture was carried out by the yeastSaccharomyces cerevisiae in the presence of a compatible xylose isomerase. The enzyme converted xylose to xylulose andS. cerevisiae fermented xylulose, along with glucose, to ethanol at pH 5.0 and 30°C. This compatible xylose isomerase fromCandida boidinii, having an optimum pH and temperature range of 4.5?5.0 and 30?50°C respectively, was partially purified and immobilized on an inexpensive, inert and easily available support, hen egg shell. An immobilized xylose isomerase loading of 4.5 IU/(g initial xylose) was optimum for SIF of xylose as well as SICF of glucose-xylose mixture to ethanol byS. cerevisiae. The SICF of 30 g/L glucose and 70 g xylose/L gave an ethanol concentration of 22.3 g/L with yield of 0.36 g/(g sugar consumed) and xylose conversion efficiency of 42.8%.
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KEYWORD
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xylose isomerase, Saccharomyces cerevisiae, Candida boidinii, xylose, ethanol fermentation, bioconversion
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