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KMID : 1094720000050020141
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 2 p.141 ~ p.145
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Insecticidal toxin fromXenorhabdus nematophilus, symbiotic bacterium associated with entomopathogenic nematodeSteinernema glaseri
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Ryu Keun-Garp
Bae Jun-Sang
Yu Yeon-Su
Park Sun-Ho
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Abstract
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Entomopathogenic nematodes are being used for insect control. We purified a toxin secreted by the insect-pathogenic bacterium,Xenorhabdus nematophilus, which lives in the gut of entomopathogenic nematodes. Culture broth ofX. nematophilus was separated by centrifugation and concentrated by ultrafiltration. The concentrated culture broth was applied to a DEAE Sephadex A-50 column, and proteins were eluted stepwise with increasing concentrations of KCl. Fractions with insect toxicity were further concentrated and then applied to a HPLC with a gel filtration column. The molecular weight of purified toxin was 39 kDa on SDS-PAGE, and Fourier transformed infrared (FTIR) spectroscopy indicated that this toxin could be a new protein exhibiting the characteristics of C=O stretching peak near 1650 cm?1.
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KEYWORD
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insecticidal toxin, Xenorhabdus nematophilus, entomopathogenic nematode, Steinernema glaseri, biological pesticide
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