|
KMID : 1094720000050020146
|
|
Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 2 p.146 ~ p.149
|
|
|
High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori
|
|
Inumaru Shigeki
Kokuho Takehiro
Yada Takashi
Kiuchi Makoto
Miyazawa Mitsuhiro
|
|
|
Abstract
|
|
|
|
Biologically active porcine Interleukin-2(poIL-2) was produced fromin vitro andin vivo baculovirus expression systems, namely theAutographa californica nuclear polyhedrosis virus (AcNPV)-cell culture system and the Hybrid nuclear polyhedrosis virus (HyNPV)? silkworm larva system. The concentration of the recombinant poIL-2(rpoIL-2) in the larvae hemolymph was 1 to 3 mg/mL, which was about 7 to 20 times those of the cell culture systems. The level of this expression efficiency is equal to that with transgenic livestock, secretion products in milk.
|
|
|
KEYWORD
|
|
|
porcine IL-2, gene expression, baculovirus, silkworm, cytokine, mass-production
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|